Possibility of detecting Erwinia in seed potatoes increased

images magazine news 2015 pw1 2015 NAK erwinia sWithin the Erwinia Delta Plan, a method has been developed that can reveal the presence of the Erwinias, Pectobacterium, and Dickeya in seed potatoes with more certainty.

images magazine news 2015 pw1 2015 NAK erwinia lWithin the Erwinia Delta Plan, a method has been developed that can reveal the presence of the Erwinias, Pectobacterium, and Dickeya in seed potatoes with more certainty. The improved method uses waste tubers that are removed from the grader and a test which examines the entire tuber. To show Erwinia in seed potatoes, the NAK (General Inspection Service for Agricultural Seed and Seed Potatoes) examines 4 mixed samples of 50 tubers from every lot. The laboratory technicians cut a slice from the navel ends of each single tuber. After grinding the navel ends, the technicians dilute the extract in a PEB medium, which boosts the growth of the Erwinias. After three days’ incubation under low-oxygen conditions, the DNA is filtered from the extracts. The following step is a test with molecular assays (TaqMan) for the presence of the various Erwinias that can cause bacterial infections.

 

Jan van der Wolf